Autologous T cells engineered ex vivo via non-viral transposon electroporation to express a PSMA-targeted chimeric antigen receptor. CAR engagement of PSMA activates T-cell signaling, proliferation, and cytotoxic killing of PSMA-positive tumor cells through perforin/granzyme release and cytokine secretion; co-expressed enhancement factors are intended to boost innate and adaptive immune activation and T-cell persistence.