A preparation of allogeneic engineered T-lymphocytes expressing a lentiviral vector encoding chimeric antigen receptor (CAR) targeting the tumor-associated antigen (TAA) CD19 and an anti-CD3 single chain antibody with the KDEL peptide fused to its C-terminus, with potential immunostimulating and antineoplastic activities. Upon administration, allogeneic anti-CD19 CAR T-cells ThisCART19A specifically target and bind to CD19-expressing tumor cells, thereby selectively lysing CD19-expressing tumor cells. CD19 antigen is a B-cell specific cell surface antigen expressed in all B-cell lineage malignancies. By eliminating TCR/CD3 expression, the potential induction of graft-versus-host disease (GvHD) by the donor T-cells is abrogated.